Table 2. Baseline gating parameters of NaChBac single-cysteine mutants.

NaChBac mutant	Activation			Inactivation
	V1/2	Z		V1/2	Z
	mV	e0		mV	e0
WT	−33.93 ± 1.26	3.34 ± 0.19		−54.08 ± 0.53	6.73 ± 0.28
V40C	−28.15 ± 1.62	3.29 ± 0.38		−47.34 ± 1.96***	6.19 ± 0.32
S129C	−41.68 ± 2.64	1.66 ± 0.17*		−77.84 ± 0.65***	3.66 ± 0.33*
T189C	−40.48 ± 1.09	4.23 ± 0.82		−58.04 ± 1.22	7.01 ± 0.33
F227C	−32.56 ± 4.36	2.18 ± 0.21		−60.84 ± 1.73**	5.07 ± 0.57*

Values are given as mean ± SEM (n = 4–12). Gating parameters of NaChBac WT versus each mutant, evaluated by one-way ANOVA with Bonferroni post hoc correction: *, P < 0.05; **, P < 0.01; ***, P < 0.0001.