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. 2018 Sep 3;215(9):2379–2395. doi: 10.1084/jem.20172168

Figure 3.

Figure 3.

ARID5B expression is elevated in adaptive NK cells. (a) Shown is a heat map of average normalized expression of genes belonging to the ARID family from the RNA-seq dataset. (b) Cumulative qRT-PCR data of ARID5B mRNA expression in the indicated canonical and adaptive NK cell subsets sorted from three HCMV-seropositive donors. All expression values were normalized against ACTB, and fold-expression values were determined for each subset relative to CD3−CD56dimCD57−NKG2C− canonical NK cells. Results are from two independent experiments. (c) Sashimi plots of RNA-seq data from a representative donor showing genomic alignments of RNA-seq reads and splice junctions within the ARID5B locus. (d) Canonical and adaptive NK cells were sorted from two seropositive donors in two independent experiments. Western blots were performed with antibodies against ARID5B and β-actin. Shown is a representative blot from one donor (left) and quantification by densitometry for both donors (right). (e) Methylation patterns throughout the ARID5B locus generated from whole-genome methylation profiling of the indicated canonical and adaptive NK cell populations sorted from four seropositive donors. Error bars represent SEM. Paired Student’s t tests were used to determine statistical significance. *P < 0.05.