Figure 5.

Knockdown of ARID5B leads to reduced survival and expression of the pro-survival protein BCL-2. (a) Cell cycle analysis of control and shARID5B NK-92 cells by propidium iodide staining (PI). Shown are representative FACS plots for both NK-92 lines (left) and cumulative data of the percentages of cells in each cell cycle phase (right). Experiments were replicated twice. (b) Control and shARID5B NK-92 cells were plated at equal numbers, and viable cell counts were taken after 2 and 3 d in culture. Shown is cumulative fold expansion data for both lines. Results are from four replicates in two independent experiments. (c) Control and shARID5B NK-92 cells were stained with annexin V and propidium iodide and analyzed by flow cytometry. Shown are representative FACS plots gated on lymphocytes (left) and cumulative fold differences (right). (d) Western blot of BCL-2 in control and shARID5B NK-92 cells. Shown is a representative blot (left) and fold decrease in shARID5B NK-92 cells calculated by densitometry (right). (c and d) Experiments were replicated twice. Error bars represent SEM. Paired Student’s t tests were used to determine statistical significance. *P < 0.05, **P < 0.01. n.s., not significant.