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. 2018 Sep 3;215(9):2429–2443. doi: 10.1084/jem.20171820

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© 2018 Singh et al.

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Figure 1. — BCAP is up-regulated in activated CD8⁺ T cells. (A) Expression of Pik3ap1 mRNA by splenic CD8⁺ OT-I T cells at the indicated times following infection with LM-OVA. Data are from the Immunological Genome Project. (B) Flow cytometry analysis of BCAP expression by CD8⁺ T cells from WT (open histograms) or Pik3ap1^−/− (filled histograms) mice at the indicated times following stimulation with plate-bound anti-CD3/anti-CD28. Data are representative of more than five independent experiments. (C) Flow cytometry analysis of CFSE dilution and CD25 expression by WT CD8⁺ T cells activated for 29 h with plate-bound anti-CD3/anti-CD28 + IL-2 (left) and BCAP expression by WT (open histograms) or Pik3ap1^−/− (filled histograms) cells in each of the indicated gates. (D) Flow cytometry analysis of BCAP and T-bet expression by WT or Pik3ap1^−/− CD4⁺ T cells activated and polarized under TH1 or TH2 conditions as indicated. (E) Flow cytometry analysis of BCAP and T-bet expression by gated naive, T_CM, T_EM, and T_EMRA CD8⁺ T cells from human peripheral blood as indicated. (C–E) Data are representative of three independent experiments.