Figure 6.

Altered CD8⁺ memory T cell development in the absence of BCAP. (A) Flow cytometry analysis of KLRG1 and CD62L expression by splenic WT and Pi3kap1^−/− OT-I T cells gated as in Fig. 4 A at the indicated times after LM-OVA infection. Data are representative of eight mice per time point that were analyzed in two independent experiments. (B) Graphical analysis of the frequencies of splenic WT and Pi3kap1^−/− OT-I T cells with the indicated KLRG1⁺T_EM, KLRG1⁻T_EM, and T_CM phenotypes at each of the indicated times after LM-OVA infection. n = 4 mice analyzed per time point in a single experiment, representative of two individual experiments. Overall statistical significance is determined by two-way paired ANOVA, and multiple comparisons were performed using Sidak’s multiple comparisons test. *, P ≤ 0.05. (C) Graphical analysis indicating the number of WT and Pik3ap1^−/− CD8⁺ T cells (mean ± SD) of the indicated phenotypes recovered from the spleens of recipient mice at the indicated times after infection. Data are representative of eight mice per time point that were analyzed in two independent experiments. (D) Graphical analysis of the frequencies of CD27⁺CXCR3⁺ cells among gated KLRG1⁺ or KLRG1⁻ splenic WT and Pi3kap1^−/− OT-I T cells 21 d after LM-OVA infection. Linked points represent values from cotransferred WT and Pi3kap1^−/− OT-I T cells recovered from individual recipient mice. Statistical significance was determined by paired Student’s t test. Data are representative of eight mice per genotype per time point that were analyzed in two independent experiments. *, P ≤ 0.05.