Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 3;217(9):3091–3108. doi: 10.1083/jcb.201712029

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Jores et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Cytosolic chaperones interact with newly synthesized β-barrel proteins. (A and B) In vitro translation reactions using yeast extracts without mRNA (Ø) or programmed with mRNA encoding DHFR-HA (D), Porin-HA (P), Tom40-HA (40), Tob55Δ120-HA (55), Om14-HA (O), Cyc3-HA (C), Tim23-HA (T), or Yah1-HA (Y) were subjected to a pull-down with anti-HA beads. Samples from the input and the eluates were analyzed by SDS-PAGE and immunodecoration with the indicated antibodies. (C) Yeast extracts programmed with mRNA encoding for Porin-HA were incubated for the indicated times. Afterward, the reactions were subjected to an anti-HA pull-down and analyzed as in A. (D) Yeast extracts were incubated with the indicated Bpa-containing peptide at the specified concentrations. Some samples were either illuminated with UV light (+UV) to induce cross-linking or were left in darkness (−UV). Then, the samples were analyzed by SDS-PAGE and immunodecoration with the indicated antibodies. PA, specific photoadducts.