Figure 2.

Differential distribution patterns of LAMP1 and cathepsin D in adult mature DRG neurons in vivo. (A and B) Representative images (A) showing partial colocalization of LAMP1 and cathepsin D in DRG axon bundles from adult mice at P120. Mice were perfused with 50% Bouin’s solution, and DRGs were isolated for immunohistochemical staining with antibodies against LAMP1 (green), cathepsin D (red), and βIII-tubulin (blue). Dashed lines show the edges of axon bundles labeled by βIII-tubulin. Arrows indicate LAMP1-labeled organelles with no detectable cathepsin D. Note that although LAMP1 signals were detectable within axon bundles as shown in the enlarged boxed area (B), only 16.62 ± 1.39% (MCC based) or 21.78 ± 2.13% (particle based) of them colocalized with detectable cathepsin D. Data were quantified from a total of 15 images of DRG axonal bundles. Bars: (A) 100 µm; (B) 20 µm.