Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 3;217(9):3127–3139. doi: 10.1083/jcb.201711083

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 8. — LAMP1 targets a heterogeneous population of endocytic organelles. (A–D) Representative images (A and B) and quantitative analysis (C and D) showing a partial colocalization of LAMP1 with Rab7-positive LEs (A and C) or Rab9-positive LEs/retromers (B and D). Mouse cortical neurons were coimmunostained at DIV7, and images were taken from axon segments. Arrows indicate LAMP1 colocalized with Rab7 or Rab9. (E and F) Representative images (E) and quantitative analysis (F) showing a partial colocalization of LAMP1 with CI-M6PR in cortical and DRG neurons (DRGNs). Embryonic mouse cortical neurons and adult DRG neurons were coimmunostained at DIV7 or 3, respectively, with antibodies against CI-M6PR, LAMP1, and MAP2 or βIII-tubulin. Images were taken from the soma (top), dendrites (middle), and DRG axons (bottom), respectively. Arrows indicate LAMP1-positive organelles that were colabeled with CI-M6PR. (G and H) Representative images (G) and quantitative analysis (H) showing a small portion of LAMP1 colocalized with the EE marker EEA1. Cortical neurons were coimmunostained at DIV7 with antibodies against EEA1 and LAMP1, followed by imaging from the soma (left), dendrites (right), and axons (not depicted). Arrows indicate colocalization of LAMP1 and EEA1 in the soma and along dendrites. Relative colocalization was assessed by MCC-based analysis. Data were quantified from the total number of neurons indicated within the bar graphs for each group from three experiments. Error bars indicate SEM. Bars, 10 µm.