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. 2018 May 14;9(5):1041–1051. doi: 10.1111/jdi.12815

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© 2018 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Proliferation of retinal neurons after transfection among five groups detected by EdU assay. (a) Detection of cell proliferation by 5‐ethynyl‐2′‐deoxyuridine (EdU) staining. (b) Cartogram of cell proliferation of each group. Red fluorescence in (a) were newly proliferated cells. *P < 0.05, compared with the negative control (NC) and blank groups; ^# P < 0.05, compared with the short hairpin ribonucleic acid plasmid (sh‐IGF‐1) group. DAPI, 4′,6‐diamidino‐2‐phenylindole; IGF‐1, insulin‐like growth factor‐1; miR‐365, micro‐ribonucleic acid‐365.