TABLE I.
Sterols Which Are Unable to Suppress TGF-β-Stimulated Luciferase Activity in Mv1Lu Cells Stably Expressing a Smad2-Dependent Luciferase Reporter Genea
| Sterol |
|---|
| Desmosterol |
| 7α-hydroxycholesterol |
| 7β-hydroxycholesterol |
| 25-hydroxycholesterol |
| 27-hydroxycholesterol |
| 7-ketocholesterol |
| 5-Cholesten-3-one |
| 5-Choesten-7-one |
| 5-Cholesten-3β-ol-7-one |
| Lanosterol |
| Lathosterol |
| Zymosterol |
a
Mv1Lu cells stably expressing a luciferase reporter gene [Chen et al., 2007, 2009] were incubated with 100 pM TGF-β in the presence of several concentrations of sterols (0, 25, and 50 μg/ml) and 7-DHC (25 μg/ml) as a positive control. After 5 h at 37°C, luciferase activity of cell lysates of treated cells was measured. TGF-β-stimulated luciferase activity in cells treated with TGF-β alone was taken as 100%. None of these sterols on the list significantly inhibited the TGF-β-stimulated luciferase activity.