Cells were seeded on coverslips and treated with vehicle only (panels A to D), 10 μM PDGF peptide (panels E to H), 10 μM VEGF peptide (panels I to L), or 100 μg/ml HA (panels M to P) for 0 min, 0.5 h, 1 h, and 2 h. After incubation, cells were fixed with methanol at −20ºC for 10 min. Cells were stained with anti-CRSBP-1 serum followed by rhodoamine red-conjugated goat anti-rabbit IgG and visualized with a fluorescence microscope. The bar indicates a scale of 10 μm (panel A). Data are representative of four independent experiments.