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Targeting of autoimmune B cells using mRBC-NPs. Leukocytes were isolated from sensitized mice exhibiting autoimmune anemia or from naïve mice. Subsequently, the cells were incubated with DiD-labeled PEG-NPs, mRBC-NPs, or mRBC-NPs blocked with anti-mRBC antibodies and analyzed by flow cytometry; a FITC-conjugated CD19 antibody was used to identify the B cell population during analysis (n = 5 for naïve, 10 for sensitized; mean ± SEM). ***p < 0.001; one-way ANOVA.
