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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Am J Transplant. 2018 Apr 10;18(10):2417–2428. doi: 10.1111/ajt.14717

Figure 3. Experimental model outlining the time course for orthotopic left lung transplants with donor BD induction.

Figure 3

Schematic presentation of experimental design. Thirty minutes prior to lung harvest mice were nebulized with either vehicle or C3aR antagonist (C3aRA). Lungs were stored for 18 hrs in Perfadex® solution at 4°C. Lungs were transplanted and harvest 6 hrs post transplantation for analysis that included BAL, qRT-PCR, and immunohistochemistry of allografts. LD were not nebulized prior to cold storage.