Table 1.

Parameters relevant for modeling the RAS activation cycle, for wildtype (WT) RAS and 15 oncogenic RAS variants. Rate constants correspond to those in Fig. 1. Variables have units s^-1 for first-order reactions and M^-1s^-1 for second-order reactions. Values are formatted for the RAS isoform for which the measurement was taken. Values for KRAS are in bold, values for HRAS are italic, and values for NRAS are underlineds

RAS Mutation	ka, GTP (1/M/s)		kd,GTP (1/s)		ka,GDP (1/M/s)		kd,GDP(1/s)		khyd (1/s)
None (WT)			2.05E-4	[37]			6.33E-5	[48]	1.47E-4	[48]
			1.00E-4	[174]			4.20E-4	[174]	3.40E-4	[174]
			2.00E-3	[175]			2.00E-3	[175]	6.80E-4	[175]
	1.40E8	[174]	9.00E-5	[176]	5.10E7	[174]	1.20E-4	[176]	2.10E-4	[176]
	2.20E6	[173]	2.50E-4	[177]	2.30E6	[173]	1.08E-4	[177]	3.49E-4	[177]
			2.33E-4	[180]			3.00E-5	[38]	9.30E-3	[38]
							2.17E-4	[180]	2.17E-4	[180]
							1.60E-5	[173]
							0.013	[181]
G12A			200E-3	[175]			2.00E-3	[175]	1.30E-5	[175]
			9.00E-5	[176]			5.00E-5	[176]	5.00E-5	[176]
G12C			2.00E-3	[175]			2.00E-3	[175]	4.90E-4	[175]
			6.00E-5	[176]			2.20E-4	[176]	1.80E-4	[176]
G12D			5.00E-4	[174]			2.00E-4	[174]	1.50E-4	[174]
			2.00E-3	[175]			2.00E-3	[175]	1.90E-4	[175]
	4.80E8	[174]	9.50E-4	[176]	7.00E7	[174]	1.60E-4	[176]	1.40E-4	[176]
	7.54E6	[30]	1.25E-3	[30]	3.16E6	[30]	5.16E-5	[30]	1.40E-4	[30]
			6.00E-4	[180]			1.67E-4	[180]	9.33E-5	[180]
G12E			4.90E-4	[176]			1.50E-4	[176]	1.60E-4	[176]
G12R			2.00E-3	[175]			2.00E-3	[175]	1.80E-5	[175]
							6.17E-3	[181]
G12S			2.00E-5	[176]			4.80E-4	[176]	1.20E-4	[176]
G12V			8.00E-5	[174]			3.33E-5	[48]	1.48E-5	[48]
	5.80E8	[174]	2.00E-3	[175]			1.30E-4	[174]	4.00E-5	[174]
	9.11E6	[30]	9.00E-5	[176]	1.16E8	[174]	2.00E-3	[175]	4.20E-5	[175]
			2.00E-4	[30]	5.23E6	[30]	2.00E-5	[176]	5.00E-6	[176]
							2.00E-5	[38]	1.50E-3	[38]
							3.35E-5	[30]	5.23E-5	[30]
							3.83E-3	[181]
G13C			8.00E-05	[176]			2.50E-4	[176]	1.10E-4	[176]
G13D			0.018	[175]			9.45E-4	[48]	5.17E-5	[48]
			6.30E-04	[176]			0.027	[175]	9.60E-5	[175]
							1.60E-4	[176]	1.90E-4	[176]
G13S			7.00E-5	[176]			3.60E-4	[176]	3.20E-4	[176]
G13V			1.20E-4	[176]			3.40E-4	[176]	2.00E-4	[176]
Q61H			2.00E-3	[175]			2.00E-3	[175]	1.30E-5	[175]
			1.53E-4	[177]			1.60E-4	[177]	3.05E-5	[177]
							0.012	[181]
Q61K			1.50E-4	[177]			1.52E-4	[177]	7.07E-4	[177]
Q61L			2.00E-3	[175]			1.50E-4	[48]	1.83E-6	[48]
			2.67E-4	[177]			2.00E-3	[175]	8.00E-6	[175]
							2.67E-4	[177]	7.58E-6	[177]
Q61P			3.17E-4	[177]			1.13E-4	[177]	2.72E-5	[177]
Q61R			1.12E-4	[177]			1.23E-4	[177]	6.10E-6	[177]
Q61W			2.00E-4	[177]			1.08E-4	[177]	2.53E-5	[177]
10G11			5.50E-4	[180]			1.18E-3	[180]	1.83E-5	[180]

^GGEF is RASGRF1. ^SGEF is SOS1. ^RGAP is RASA1. ^NGAP is NF1. *calculated from Lenzen et al. [173] using nucleotide dissociation rate as k_r = 3.9 s^-1 = k_d,GDP,GEF and K_d (K_d = K_d4), such that k_f = k_a,GTP,GEF = k_r/K_d. **calculated from Lenzen et al. [173], k_r = K_d3k_f3 = (0.6e-3)(3.4e4), assuming the same dissociation rate of GEF from RAS-GTP and RAS-GDP.