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LAMP-2C ectopic expression in melanoma cells altered LAMP-2A and LAMP-2B protein expression. LAMP-2C myc, total LAMP-2, LAMP-2A, LAMP-2B, HSC70, HSP90, and actin were detected in DM331 pCMV and DM331 2C myc cells. The normalized expression of each protein was set equal to one for DM331 pCMV cells for relative comparison with protein levels in DM331 2C myc cells. Data were analyzed by two-tailed, unpaired Student’s t-test. ∗p < 0.05 and ∗∗∗p < 0.001 (n = 3).
