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. 2018 Aug 29;9:977. doi: 10.3389/fphar.2018.00977

FIGURE 2.

FIGURE 2

Effect of PIO on BKCa channel activity in mHippoE-14 hippocampal neurons. (A) Original current traces of BKCa channels obtained in the absence (left) and presence (right) of 10 μM pioglitazone (PIO). The examined cells were bathed in symmetrical K+ solution (145 mM). Under inside-out current recordings, the potential was held at +60 mV and bath medium contained 0.1 μM Ca2+. The upward deflection represents the opening event of the channel. The lower part indicates the expanded trace recorded from the uppermost part in the control and during exposure to PIO. (B) BKCa-channel trace obtained after washout of PIO. (C) Concentration-dependent increase in channel open probability (mean ± SEM; n = 9–11 for each point). Channel activity measured at +60 mV during the exposure to 100 μM PIO was taken to be 100%. The values for EC50, Hill coefficient and maximal percentage increase of BKCa channels in the presence of PIO were 7.6 μM 1.3 and 100%, respectively.