Table 2.

MS/MS data in the positive and negative ESI modes and the identification results for the biomarkers.

Peak no.	RT (min)	Mode	m/z	Formula	Identification	Significance	Content variance	Percentage change	Reported	Metabolic pathway
1	6.07	[M + H]+	568.3423	C26H50NO10P	Phosphatidylserine	0.2332	↓	33.73	Yes	Glycerophospholipid metabolism
2	8.19	[M + H]+	274.2722	C16H35NO2	C16-Sphingosine	0.4179	↓	12.85	Yes	Sphingolipid metabolism
3	8.70	[M + H]+	318.2017	C21H35NO	N-methyl arachidonic amide	0.1240	↑	144.8	No	Arachidonic acid metabolism
4	8.80	[M + H]+	362.3266	C23H39NO2	N-(2-methoxyethyl) arachidonic amide	0.1830	↓	52.14	No	Arachidonic acid metabolism
5	16.72	[M + H]+	520.3378	C26H50NO7P	Linoleamidoglycerophosphate choline	0.1129	↓	17.73	No	Glycerophospholipid metabolism
6	16.76	[M + HCOO]-	564.3354	C27H51NO9P	Lyso-PC (C18:2)	0.1836	↓	42.28	Yes	Glycerophospholipid metabolism
7	18.59	[M + H]+	496.3356	C24H50NO7P	Lyso-PC (C16:0)	0.0947	↓	32.22	Yes	Glycerophospholipid metabolism
8	19.61	[M + HCOO]-	566.3452	C27H53NO9P	Lyso-PC (C18:1)	0.0854	↓	85.12	Yes	Glycerophospholipid metabolism
9	22.37	[M-H]-	303.2318	C20H32O2	Arachidonic acid	0.3225	↑	299.42	Yes	Arachidonic acid metabolism
10	22.52	[M-H]-	279.2293	C18H32O2	Linoleic acid	0.2874	↓	70.48	Yes	Fatty acid β-oxidation metabolism