Table 1.
Section | Sample name | Cultivar | Sampling location | Origin | Technique | Sampling date | GaTLV | GAPDH |
---|---|---|---|---|---|---|---|---|
1st observation | EVC53 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | Illumina 2*150 | 05/09/2013 | ✓ | Na |
EVC42 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | Illumina 2*150 | 05/09/2013 | ✓ | Na | |
EVC60 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | Illumina 2*150 | 05/09/2013 | ✓ | Na | |
EVC56 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | Illumina 2*150 | 05/09/2013 | ✓ | Na | |
Epidemiology linked to timing | V. sylvestris | Na | Alsace-Colmar-INRA-open field | Grau du roi, Fr | RT-PCR | 18/05/2011 | ✗ | NT |
V. rupestris | Na | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 17/06/2008 | ✗ | NT | |
V. candicans | Na | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 17/06/2008 | ✗ | NT | |
V. ishikari | Na | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 17/06/2008 | ✗ | NT | |
V. davidii | Na | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 17/06/2008 | ✗ | NT | |
V. amurensis | Na | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 17/06/2008 | ✗ | NT | |
A. japonica | Na | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 17/06/2008 | ✗ | NT | |
A. cordata | Na | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 17/06/2008 | ✗ | NT | |
A. peunculata | Na | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 17/06/2008 | ✗ | NT | |
Y276 | Otscha Bala | Alsace-Colmar-INRA-open field | Ouzbekistan | RT-PCR | 06/08/2012 | ✗ | NT | |
95-184 | Grand Noir | Alsace-Colmar-INRA-open field | Volos, Greece | RT-PCR | 11/09/2014 | ✓ | NT | |
P119 | Zirock | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 12/09/2014 | ✓ | NT | |
C1200 | Chardonnay | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 22/09/2014 | ✓ | NT | |
N37 | Cabernet franc | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 22/09/2014 | ✓ | NT | |
96-29 | Lambrusco Lambo | Alsace-Colmar-INRA-open field | Italy | RT-PCR | 26/09/2012 | ✓ | NT | |
Z170 | Ruby Cabernet | Alsace-Colmar-INRA-open field | France | RT-PCR | 26/09/2012 | ✓ | NT | |
96-60 | Nebbiolo | Alsace-Colmar-INRA-open field | Italy | RT-PCR | 26/09/2012 | ✓ | NT | |
Y245 | Karasakis | Alsace-Colmar-INRA-open field | Turquie | RT-PCR | 27/09/2012 | ✓ | NT | |
96-42 | Nebbiolo | Alsace-Colmar-INRA-open field | Italy | RT-PCR | 02/10/2012 | ✓ | NT | |
Y259 | Lutea | Alsace-Colmar-INRA-open field | Italy | RT-PCR | 02/10/2012 | ✓ | NT | |
Y270 | Muscat d′Istambul | Alsace-Colmar-INRA-open field | Israël | RT-PCR | 02/10/2012 | ✓ | NT | |
E4 | Redglobe | Alsace-Colmar-INRA-open field | IFV-ENTAV | RT-PCR | 24/10/2012 | ✓ | NT | |
Y206 | Chaouch rose | Alsace-Colmar-INRA-open field | Turkey | RT-PCR | 25/10/2011 | ✗ | NT | |
BB2-6 | Touriga Francesa | Alsace-Colmar-INRA-open field | Portugal | RT-PCR | 25/10/2011 | ✗ | NT | |
95-151 | Alphonse Lavallée | Alsace-Colmar-INRA-open field | IFV-ENTAV | RT-PCR | 29/10/2012 | ✓ | NT | |
T113 | Chatus | Alsace-Colmar-INRA-open field | Bordelais | RT-PCR | 09/11/2011 | ✓ | NT | |
Timing confirmation | EVC53 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 20/06/2016 | ✗ | NT |
EVC53 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 11/07/2016 | ✗ | ✓ | |
EVC53 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 29/08/2016 | ✗ | NT | |
EVC53 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 22/09/2016 | ✓ | ✓ | |
EVC60 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 20/06/2016 | ✗ | NT | |
EVC60 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 11/07/2016 | ✗ | ✓ | |
EVC60 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 29/08/2016 | ✗ | NT | |
EVC60 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 22/09/2016 | ✓ | ✓ | |
Tu1 | Nd | Alsace-Turckheim-Abandonned | Nd | RT-PCR | 30/05/2017 | ✗ | NT | |
Tu2 | Nd | Alsace-Turckheim-Abandonned | Nd | RT-PCR | 30/06/2017 | ✗ | NT | |
Tu3 | Nd | Alsace-Turckheim-Abandonned | Nd | RT-PCR | 30/07/2017 | ✓ | NT | |
Win1 | Nd | Alsace-Wintzenheim-ResDur | Nd | RT-PCR | 30/05/2017 | ✗ | NT | |
Win2 | Nd | Alsace-Wintzenheim-ResDur | Nd | RT-PCR | 30/06/2017 | ✗ | NT | |
Win3 | Nd | Alsace-Wintzenheim-ResDur | Nd | RT-PCR | 30/07/2017 | ✓ | NT | |
Open field vs. Greenhouse | EVC49 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 22/09/2016 | ✓ | ✓ |
EVA4 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 22/09/2016 | ✓ | ✓ | |
EVA13 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 22/09/2016 | ✓ | ✓ | |
EVA16 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 22/09/2016 | ✓ | ✓ | |
EVA20 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 22/09/2016 | ✓ | ✓ | |
EVA24 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 22/09/2016 | ✓ | ✓ | |
EVA49 | Gewurztraminer | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 22/09/2016 | ✓ | ✓ | |
A | Nd | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 27/09/2016 | ✓ | ✓ | |
B | Nd | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 27/09/2016 | ✓ | ✓ | |
C | Nd | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 27/09/2016 | ✓ | ✓ | |
D | Nd | Alsace-Colmar-INRA-open field | Nd | RT-PCR | 27/09/2016 | ✓ | ✓ | |
Kober 5BB healthy | Na | Alsace-Colmar-INRA-Greenhouse | Grau du roi, Fr | RT-PCR | 27/09/2016 | ✓ | ✓ | |
Kober 5BB GFLVinf1 | Na | Alsace-Colmar-INRA-Greenhouse | Grau du roi, Fr | RT-PCR | 27/09/2016 | ✗ | ✓ | |
Kober 5BB GFLVinf2 | Na | Alsace-Colmar-INRA-Greenhouse | Grau du roi, Fr | RT-PCR | 27/09/2016 | ✗ | ✓ | |
Kober 5BB GFLVinf3 | Na | Alsace-Colmar-INRA-Greenhouse | Grau du roi, Fr | RT-PCR | 27/09/2016 | ✓ | ✓ | |
Gw cl643 | Gewurztraminer | Alsace-Colmar-INRA-Greenhouse | Grau du roi, Fr | RT-PCR | 27/09/2016 | ✗ | ✓ | |
Chard cl131 | Chardonnay | Alsace-Colmar-INRA-Greenhouse | Grau du roi, Fr | RT-PCR | 27/09/2016 | ✗ | ✓ | |
ENTAV-E39 | 11R-cl237 | Alsace-Colmar-INRA-Greenhouse | Grau du roi, Fr | RT-PCR | 27/09/2016 | ✗ | ✓ | |
ENTAV-E173 | Grenache-cl435 | Alsace-Colmar-INRA-Greenhouse | Grau du roi, Fr | RT-PCR | 27/09/2016 | ✗ | ✓ | |
ENTAV Kober 5BB cl114 | Na | Alsace-Colmar-INRA-Greenhouse | Grau du roi, Fr | RT-PCR | 27/09/2016 | ✗ | ✓ | |
Cramant N332 | Chardonnay | Alsace-Colmar-INRA-Greenhouse | Cramant, Fr | RT-PCR | 27/09/2016 | ✗ | ✓ | |
Bennwhir BE/5-47 N79 | Gewurztraminer | Alsace-Colmar-INRA-Greenhouse | Bennwhir, Fr | RT-PCR | 27/09/2016 | ✗ | ✓ | |
Chablis 39-1 N24 | Chardonnay | Alsace-Colmar-INRA-Greenhouse | Chablis, Fr | RT-PCR | 27/09/2016 | ✗ | ✓ | |
Epidemiology | E | Nd | Alsace-Eguisheim | Nd | RT-PCR | 27/09/2016 | ✓ | ✓ |
F | Nd | Alsace-Husseren les Chateaux | Nd | RT-PCR | 27/09/2016 | ✓ | ✓ | |
G | Nd | Alsace-Voegtlinshoffen | Nd | RT-PCR | 27/09/2016 | ✓ | ✓ | |
H | Nd | Alsace-Gueberschwihr | Nd | RT-PCR | 27/09/2016 | ✓ | ✓ | |
I | Nd | Alsace-Grand cru Hatschbourg | Nd | RT-PCR | 27/09/2016 | ✓ | ✓ | |
J | Nd | Alsace-Herrlisheim | Nd | RT-PCR | 27/09/2016 | ✓ | ✓ | |
Mes | Nd | Cognac-Messac | Nd | RT-PCR | 01/11/2016 | ✗ | ✓ | |
Ger | Nd | Cognac-Germignac | Nd | RT-PCR | 01/11/2016 | ✗ | ✓ | |
Sal | Nd | Cognac-Salignac | Nd | RT-PCR | 01/11/2016 | ✗ | ✓ | |
TT2017-73 | Nd | Burgundy-Villers la Faye | Nd | Illumina 2*150 | 11/09/2017 | ✓ | Na | |
TT2017-74 | Nd | Burgundy-Villers la Faye | Nd | Illumina 2*150 | 11/09/2017 | ✓ | Na | |
TT2017-75 | Nd | Burgundy-Villers la Faye | Nd | Illumina 2*150 | 11/09/2017 | ✓ | Na | |
TT2017-76 | Nd | Burgundy-Villers la Faye | Nd | Illumina 2*150 | 11/09/2017 | ✓ | Na | |
TT2017-77 | Nd | Burgundy-Villers la Faye | Nd | Illumina 2*150 | 11/09/2017 | ✓ | Na | |
TT2017-78 | Nd | Burgundy-Villers la Faye | Nd | Illumina 2*150 | 11/09/2017 | ✗ | Na | |
TT2017-79 | Nd | Burgundy-Villers la Faye | Nd | Illumina 2*150 | 11/09/2017 | ✓ | Na | |
TT2017-80 | Nd | Burgundy-Villers la Faye | Nd | Illumina 2*150 | 11/09/2017 | ✓ | Na | |
Superficial | Swab EVC53 | Na | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 27/09/2016 | ✓ | ✗ |
Swab EVC60 | Na | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 27/09/2016 | ✗ | ✓ | |
Swab EVC56 | Na | Alsace-Colmar-INRA-open field | Colmar, Fr | RT-PCR | 27/09/2016 | ✓ | ✓ | |
Negative Swab | Na | Na | Na | RT-PCR | 27/09/2016 | ✓ | ✓ | |
Fungi | Alternaria tenuissima | Gewurztraminer | Alsace-Colmar-INRA-open field | Na | RT-PCR | 27/09/2016 | ✗ | Na |
Leptosphaerulina chartarum | Gewurztraminer | Alsace-Colmar-INRA-open field | Na | RT-PCR | 27/09/2016 | ✗ | Na | |
Epicoccum nigrum | Gewurztraminer | Alsace-Colmar-INRA-open field | Na | RT-PCR | 27/09/2016 | ✗ | Na | |
Aureobasidium pullulans | Gewurztraminer | Alsace-Colmar-INRA-open field | Na | RT-PCR | 27/09/2016 | ✗ | Na | |
Cladosporium cladosporioides | Gewurztraminer | Alsace-Colmar-INRA-open field | Na | RT-PCR | 27/09/2016 | ✗ | Na | |
Dothioraceae sp | Gewurztraminer | Alsace-Colmar-INRA-open field | Na | RT-PCR | 27/09/2016 | ✗ | Na | |
Penicillium oxalicum | Gewurztraminer | Alsace-Colmar-INRA-open field | Na | RT-PCR | 27/09/2016 | ✗ | Na | |
Cladosporium sp. | Gewurztraminer | Alsace-Colmar-INRA-open field | Na | RT-PCR | 27/09/2016 | ✗ | Na | |
Fusarium equiseti | Gewurztraminer | Alsace-Colmar-INRA-open field | Na | RT-PCR | 27/09/2016 | ✗ | Na | |
Cryptococcus magnus | Gewurztraminer | Alsace-Colmar-INRA-open field | Na | RT-PCR | 27/09/2016 | ✗ | Na | |
Botrytis cinerea | Gewurztraminer | Alsace-Colmar-INRA-open field | Na | RT-PCR | 27/09/2016 | ✗ | Na | |
Mucor circinelloides | Gewurztraminer | Alsace-Colmar-INRA-open field | Na | RT-PCR | 27/09/2016 | ✗ | Na | |
Plasmopara viticola | Chardonnay | Alsace-Colmar-INRA-open field | Na | RT-PCR | 2006 | ✗ | Na | |
Erysiphe necator | Na | Alsace-Colmar-INRA-open field | Na | RT-PCR | 2016 | ✗ | Na | |
Guignardia bidwellii | Na | Alsace-Colmar-INRA-open field | Na | RT-PCR | 2015 | ✗ | Na |
Relevant information relative to samples used in this study are shown, comprising: section (describing the purpose of sample use), names, cultivars, and sampling location, origin of the sample, techniques used to detect GaTLV, sampling dates. Na, Not applicable; Nd, Not determined; NT, Not tested; ✓: detected, ✗: absence.