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. 2018 Sep 4;8:13203. doi: 10.1038/s41598-018-31566-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time-course changes in ACT activities and HvACT1 expression in ACT transformants cultured under PR, LG1, and LG2 conditions. ACT activities catalysing the formation of pCA (●) and FA (○) in the crude extracts were measured. HvACT1 transcripts and HvACT1 protein were detected by RT-PCR (labelled mRNA) and immunoblot (labelled protein) analyses, respectively. (a) ACT-line 15 (b) ACT-line 22. Data are means ± SDs (n = 3). One katal (kat) of enzyme activity was defined as the amount of enzyme that catalyses the formation of the reaction product at a rate of 1 mol/s. Full-length gels for RT-PCR analysis and full-length blots for immunoblot analysis are presented in Supplementary Figs S6 and S7, respectively.