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. 2018 Sep 4;3:14. doi: 10.1038/s41536-018-0052-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — The expression of alveolar progenitor markers in AEC-II-iPL cells. Confocal microscopic images depict the expression of Hopx and SPC in: a adult lung; b colonies derived from AEC-II cells after a 2-week culture period in the absence of Dox (2W^ND); c E15.5 lungs; d passaged 2W^ND colonies induced with Dox for 2 weeks (2W^ND + 2W^+D); e colonies were passaged and cultured without Dox for 2 subsequent weeks (2W^ND + 2W^+D + 2W^−D), with nuclear stain DAPI (blue), SPC (green), and Hopx (red). f Quantification of each cell type (SPC single positive; Hopx single positive; SPC and Hopx double positive) in colonies obtained from different groups. g Flow cytometric analysis of integrin α6 and β4 expression levels in day 0 freshly isolated AEC-II cells and 2W^ND + 2W^+D cells. Confocal microscopic images of colonies derived from AEC-II cells after 2-week culture without Dox (2W^ND) h and 2W^ND + 2W^+D colonies (2W^ND + 2W^+D) i, showing nuclear stain DAPI (blue), β4 (green), and EpCAM (red). Confocal microscopic images depicting the co-expression of Hopx and SPC in 2W^ND + 2W^+D cells j and E15.5 lungs k, with nuclear stain DAPI (blue), β4 (green), and Hopx (red). l Cartoon depicting the generation of iPL cells using interrupted reprogramming. For f, values are mean ± S.D. of three independent biological replicates. In g, data are representative of a minimum of three independent biological replicates. Scale bar, 10 µm (a–e, h—zoom, i–k), 100 µm (h—left panel)