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. 2018 Sep 4;8:13212. doi: 10.1038/s41598-018-31594-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of reactive oxygen species (ROS) production and mitochondrial function in hUCB-EPCs treated with Ac4ManNAz. (A) hUCB-EPCs were treated with 0, 10, 20, or 50 μM Ac4ManNAz, and intracellular ROS levels were then measured using the fluorescent probe 2′, 7′-dichlorfluorescein-diacetate (DCFH-DA). (B) Changes in mitochondrial membrane potential (ΔΨm) were measured in hUCB-EPCs using the fluorescent probe JC-1. (C) Quantification of ROS generation are expressed as the percentage of fluorescence intensity relative to the control. (D) Value of mitochondrial membrane potential were expressed as % Green/Red fluorescence cells.