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. 2018 Sep 4;8:13192. doi: 10.1038/s41598-018-31575-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Live Triple-fluorescence in OVK18 cells treated with paclitaxel plus BKM120. Live OVK18 cells, control and treated were stained with MitoView Blue + NucView488 casp3 substrate +CF 594 Annexin V (A–D). Photomicrographs were taken from live cells in culture within a span of 10 minutes. In separate experiments, the validation of the effect of paclitaxel (2.5 nM) plus BKM120 (1 µM) was tested by real-time proliferation (E, left panel), apoptosis (E, right panel), the mitochondrial potential (F), and expression of apoptotic markers by WB (G). The expression of apoptotic markers by WB was semi-quantified using Image-J and presented in a bar diagram with arbitrary units. The cleaved caspase3 activity, annexin V positivity and mitochondrial status of non-treated (H) and treated (I) live OKK18 cells at 48 hours are presented. A representative of uncropped blot representing the expression of proteins (cleaved PARP1, cleaved caspase3, BIM, and beta-actin) by WB from an independent experiment is presented in the Supplementary Figure.