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. 2018 Sep 4;8:13192. doi: 10.1038/s41598-018-31575-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Live Triple-fluorescence in A2780 cells treated with paclitaxel. Live A2780 cells, control and treated were stained with MitoView Blue + NucView488 casp3 substrate +CF 594 Annexin V (A,B). Photomicrographs were taken from live cells in culture within a span of 10 minutes. In separate experiments, the validation of the effect of paclitaxel (5 nM) was tested by real-time proliferation (C, left panel), apoptosis (C, right panel), the mitochondrial potential (D), expression of apoptotic markers by WB (E) and staining of live-dead cells (F,G). A representative of uncropped blot representing the expression of proteins (cleaved PARP1, cleaved caspase3, BIM and beta-actin) by WB from an independent experiment is presented in the Supplementary Figure.