FIG 3 .

L. paracasei (Lp) and E. coli (Ec) impair TG secretion and lipid metabolism of cultured enterocytes. m-ICcl2 cells were polarized on transwell inserts for 14 to 21 days before infection of the upper compartment with bacteria (multiplicity of infection [MOI] 100). Supernatants and cell lysates were recovered after 16 h of infection. Control: noninfected cells. (A) Intracellular and supernatant TG levels. Results are normalized to control data and expressed as means ± SEM. (B and C) mRNA levels of genes involved in host lipid metabolism, including (B) PPAR-controlled genes and (C) SREBP-1c targets, assessed by RT-qPCR using Actin as reporter gene. Results are expressed as means of fold change ± SEM relative to the control. (D) Western blot analysis of SREBP-1 (3 experiments were performed in duplicate). Representative sample blots of total cell lysates show the cytoplasmic precursor (p125) and the nuclear form (p68) of SREBP-1 protein. (A to C) Statistical significance is expressed relative to the control unless otherwise specified. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (one-way ANOVA). A total of ≥3 experiments were performed in triplicate.