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. 2018 Sep 4;9(5):e01464-18. doi: 10.1128/mBio.01464-18

FIG 1 .

FIG 1 

Microfluidic single-cell cultivation and analysis of B. subtilis. (A) Time-lapse image series of B. subtilis cells harboring transcriptional fusions of Phag-cfp (motility genes) and PtapA-yfp (biofilm genes). Wild-type cells (GP2130) and isogenic ΔymdB mutant cells (GP2551) grown in LB medium at 37°C in microfluidic chambers are shown in the upper and lower panels, respectively, as indicated. Black arrows indicate the appearance of suppressor mutants that regained the ability to express biofilm genes. (B) Ratio of cell types at each time point of the time-lapse image series. n, total number of cells whose expression was determined at each time point.