Table 2.
Identification of samples | Real-time PCR/Sequencing | PCR/LDR* | Additional virus detected by PCR/LDR | ||
---|---|---|---|---|---|
No. Positive | No. Negative | No. Positive | No. Negative | ||
Rotavirus | 148 | 0 | 144 | 4 | 2† |
Sapovirus | 12 | 0 | 10 | 2 | |
Norovirus GI | 2 | 0 | 1 | 1 | |
Norovirus GII | 48 | 0 | 48 | 0 | |
Negative | 0 | 86 | 0 | 86 |
*Sensitivity and specificity of the PCR/LDR assay for detection of viruses in clinical stool specimens were 97% and 100%, respectively. †Norovirus GGII detected in addition to rotavirus by PCR/LDR in Ghanaian samples were confirmed by sequencing.