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. 2018 Sep 4;8:13182. doi: 10.1038/s41598-018-31352-2

Table 1.

The primers and enzymes used for genes cloning and RT-PCR.

Gene Name PRIMER (5′-3′) Expression Vector pDs-RED N1
SLCO1B1-F GTAAGCTTGGACCAAAATCAACATTTG HinIII
SLCO1B1-R AACTCGAGACACAATGTGTTTCACTATC XhoI
SLC22A1-F GTGGTACCATGCCCACCGTGGATGAC KpnI
SLC22A1-R AACTCGAGTTGGTGCCCGAGGGTTCTGAG XhoI
GAPDH F (RT-PCR) CTCTGCTCCTCCTGTTCGAC NA
GAPDH R (RT-PCR) GCCCAATACGACCAAATCC NA
SLCO1B1 F (RT-PCR) CTTCAAATACGTAGAGCAACAGT NA
SLCO1B1 R (RT-PCR) GTAAAAGGACAATGACATCACAG NA
SLC22A1 F (RT-PCR) TCCTGGGAACTGTGCTGG NA
SLC22A1 R (RT-PCR) CAGTTGCCCTTGCTGACC NA