The absence of lymphotoxin on RORγt expressing cells results in splenomegaly. (a) Conditional LTβR-deficient mice were generated by crossing Ltbr
f/f mice with LysM
Cre+ mice and Tie2
Cre+ mice (n=3–4, repeated two times). (b, c) Ltb
f/f mice (n=3–13, repeated five times) were bred to Cd4
Cre+ and Cd19
Cre+mice to inactivate LT in T cells and B cells, respectively. Mice were killed, and the spleens were removed and weighed. Error bars represent the mean and s.e.m. One-way ANOVA was performed. **P<0.01; ***P<0.001.