Figure 4.

Lymphotoxin expression on ILC3s prevents the development of splenomegaly. Rorc ^Cre+ Ltb ^f/f mice were crossed onto B6 Rag1 ^−/− background. The mice were killed and the spleens were harvested and (a) weighed, (b) counted, and (c) percentage and (d) absolute cell number of neutrophils were determined and compared against spleens from B6 Rag1 ^−/− mice. (e) Example flow plots showing the Gr1+CD11b+ population that has been gated on CD45+, CD3− and B220− populations from the spleen of Rag1 ^−/− and Rorc ^Cre+ Ltb ^f/f Rag1 ^−/− mouse, respectively. n=8, repeated two times. Error bars represent the mean and s.e.m. Unpaired t-tests were performed. **P<0.01; ***P<0.001; ****P<0.0001.