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. 2018 Sep 4;8:13204. doi: 10.1038/s41598-018-31604-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of the postnatal development of the OT compartments. The OT was divided into four cortex-like domains, cap compartment, superficial Islands of Calleja (ICj), and deep ICj. In each compartment, the density of NeuroTrace (+) cells (A,E), intensity of DARPP-32 immunoreactivity (B,F), intensity of D1 mRNA signal (C,G), and intensity of D2 mRNA signal (D,H) are shown. (A–D) Quantification in cortex-like domains. (E–H) Quantification in cap compartment and ICj. Data represent the mean ± SEM. Statistical significance was calculated using one-way ANOVA with post hoc Tukey’s test to compare signal intensity among the four cortex-like domains (n = 3). ^*p < 0.05; ^**p < 0.01; ^***p < 0.001.