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. 2018 Sep 4;8:13204. doi: 10.1038/s41598-018-31604-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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c-fos activation in the individual OT compartments following access and consumption of food pellets in postnatal mice. c-fos mRNA expression after 30 min of food pellet presentation was examined in individual compartments of the OT at P8, P15, P21, P27, and P56. (A) Density of c-fos (+) cells in the cortex-like domains. Statistical significance was calculated using two-way ANOVA with post hoc Tukey’s test (n = 4). (B) Density of c-fos (+) cells in the cap compartment and Islands of Calleja. Statistical significance was calculated using an unpaired t-test for P8, P21, P27, and P56, and one-way ANOVA for P15 (n = 4). Black circles, control mice; green squares, mice that were delivered with pellets but did not access or eat; orange triangles, mice that ate delivered pellets. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001; ns, not significant.