Fig. 6.

YTHDF2 KD facilitates expansion of human cord blood functional long-term HSCs. a Experimental scheme for measuring frequency of HSCs after in vivo expansion. b Representative flow plots of hCD45⁺ GFP⁺ reconstitution from primary recipient mice receiving the highest two cell doses. hCD45, human CD45 (c) hCD45⁺ GFP⁺ engraftment in BM from the primary recipient mice that received the highest two doses (n = 8). d HSC frequency determined by primary LDA. Dashed lines indicate 95% confidence intervals. e Representative flow plots of hCD45⁺ GFP⁺ reconstitution from secondary recipient mice receiving the highest two cell doses. f hCD45⁺ GFP⁺ engraftment in BM from secondary recipient mice that received the highest two doses (n = 6). g HSC frequency determined by secondary LDA. Dashed lines indicate 95% confidence intervals. Data shown as mean ± SEM. Unpaired t-test. n.s. nonsignificant