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. 2018 Aug 14;1:112. doi: 10.1038/s42003-018-0119-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Plots showing the richness assessed via each taxonomic method. a Total richness measured in all sampled sites from each phylum. b–h Richness for each taxon at each investigated site, including Annelida (b), Gastrotricha (c), Mollusca (d), Nematoda (e), Nemertea (f), Platyhelminthes (g), and Xenacoelomorpha (h). Morphotypes (black line) are identified with comparative morphology; evolutionary independent entities (red line) are recovered via DNA taxonomy (GMYC model) from 18S rRNA sequences (V1–V2 regions) of single specimens; OTUs (pink line) recovered via 18S rRNA sequences (V1–V2 regions) of single specimens; eOTUs (dark blue line), recovered via metabarcoding 18S rRNA (V9 region); sequence variants (light blue line), recovered via metabarcoding 18S rRNA (V9 region)