Table 3.
Apoptosis rate and cell cycle distribution of skeletal muscle cells among seven groups
| Group | Apoptosis rate (%) | G0/G1 (%) | S (%) | G2/M (%) |
|---|---|---|---|---|
| Control | 6.45 ± 0.51 | 71.35 ± 5.12 | 18.84 ± 1.13 | 9.81 ± 0.81 |
| Blank | 3.36 ± 0.32* | 53.37 ± 3.27 | 35.72 ± 2.41 | 10.91 ± 1.02 |
| NC | 3.47 ± 0.31* | 53.61 ± 3.62 | 34.67 ± 2.71 | 11.72 ± 1.11 |
| MBNL1AS1 vector | 5.21 ± 0.49*# | 62.94 ± 4.71*# | 27.14 ± 1.73*# | 9.92 ± 0.93 |
| si-MBNL1AS1#1 | 1.28 ± 0.11*# | 45.42 ± 3.84*# | 43.25 ± 3.61*# | 11.33 ± 1.12 |
| si-MBNL1AS1#2 | 1.22 ± 0.23*# | 43.33 ± 2.47*# | 43.33 ± 2.47*# | 43.33 ± 2.47*# |
| si-KCNMA1#1 | 5.33 ± 0.48*# | 63.16 ± 5.12 | 26.33 ± 1.73 | 10.51 ± 1.01 |
| si-KCNMA1#2 | 4.97 + 1.1*# | 61.89 ± 3.44 | 25.08 ± 1.23 | 10.34 ± 1.45 |
| si-MBNL1AS1 + si-KCNMA1 | 3.42 ± 1.31* | 58.72 ± 4.22 | 31.15 ± 2.63 | 10.13 ± 1.03 |
MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily M alpha 1, si small interfering, NC negative control
*p < 0.05 vs. the control group; # p < 0.05 vs. the blank and NC groups; measurement data were expressed as mean ± standard deviation and comparisons among multiple groups were assessed by one-way ANOVA; the experiment was repeated for 3 times