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. 2018 Jul 10;19(9):e45235. doi: 10.15252/embr.201745235

Figure 1. A post‐translational modification alters priming of mitochondria from cancer cell lines.

Figure 1

  1. Mitochondria isolated from the indicated cell lines were incubated with Bad peptide and MOMP measured as % cytochrome c release determined by ELISA. Bars indicate the mean of three independent experiments (n = 3). Symbols indicate the mean of at least two technical replicates for each independent experiment.
  2. Cells were treated with indicated concentrations of ABT‐737 for 48 h. ABT‐737 EC50 values of cancer cell lines were determined by using MTT assay (mean ± SEM, n = 4 experimental replicates).
  3. Mitochondria isolated from the indicated cell lines were incubated with the indicated BH3 peptides in the presence or absence of phosphatase inhibitor cocktail PhosSTOP in all buffers. MOMP was measured as % cytochrome c release determined by ELISA. Bars indicate the mean of three independent experiments (n = 3). Symbols indicate the mean of at least two technical replicates for each independent experiment. One‐way ANOVA was used followed by post hoc t‐tests with Bonferroni correction for multiple comparisons (ns = not significant). MDA‐MB‐468 (Bim ****P < 0.0001; Bad **P = 0.0059; BMF ***P = 0.0002). ZR‐75‐1 (Bid ***P = 0.0003; Bim ****P < 0.0001; Bad **P = 0.0041; Puma ***P = 0.0007).