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. 2018 Jul 17;19(9):e45253. doi: 10.15252/embr.201745253

Figure EV1. PAR‐3 KO does not exhibit overt defects on adherens junction formation and apical–basal polarization in the retinal vasculature.

Figure EV1

  1. Staining of control and Pard3 iΔEC P6 mice retina with adherens junction marker (VE‐cadherin) and endothelial cell marker isolectin‐B4 (iB4).
  2. Staining of control and Pard3 iΔEC P6 retina with basement membrane marker (collagen IV), apical membrane marker (podocalyxin), and isolectin‐B4 (iB4).
  3. Quantification of the number of sprouts/10 μm in angiogenic front. Data are presented as mean ± SEM (n = 3 retinas). Difference **P < 0.01, analyzed by Student's t‐test.
  4. Staining of control and Pard3 iΔEC P6 retina artery with basement membrane marker (collagen IV), apical membrane marker (podocalyxin), adherens junction marker (VE‐cadherin), and nuclei (Hoechst 33342, Sigma). 3D‐reconstituted cross‐section images from the region indicated with white dashed lines are shown in the images.
Data information: Scale bars: 100 μm (A and B, lower magnification), 25 μm (A and B, higher magnification), and 10 μm (D).