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. 2018 Jul 18;19(9):e45775. doi: 10.15252/embr.201845775

Figure 3. The UC1 domain of cavin1 is important for caveola function in vivo .

Figure 3

  1. Schematic representation of UC1 and HR2 domain deletions in cavin1.
  2. GFP‐tagged WT cavin1, Δ2 UC1 cavin1, ΔHR2 cavin1 were expressed in A431 cells and co‐stained with CAV1 antibody (red), scale bar = 10 μm.
  3. GFP‐tagged cavin1b full‐length and ∆5 UC1 cavin1b expression in 60 and 90 hpf live cavin1b −/− embryos. Scale bar = 100 μm.
  4. Live bright field images of uninjected cavin1b −/− zebrafish, and cavin1b −/− zebrafish injected with zcavin1b and ∆5 UC1 zcavin1b. 60 hpf and 90 hpf timepoints shown. Embryos at 60 hpf were electrically stimulated (10 min) and imaged at 90 hpf. Injected WT zebrafish presented no observable lesions even upon electrical stimulation (n = 75, clutch‐N = 3). Arrowheads indicate lesions. “S” next to arrowhead denotes a severe lesion. Scale bar = 100 μm.
  5. Plot of lesion number for 60 hpf embryos, 90 hpf embryos and 90 hpf embryos electrically stimulated at 60 hpf for 10 min. For 60 hpf, 90 hpf and 90 hpf stimulated; fish number (uninjected) = 84, 43, 39 respectively, fish number (zcavin1b‐GFP) = 41, 18, 24 respectively, fish number (∆5 UC1 zcavin1b‐GFP) = 52, 22, 28, respectively. Ordinary one‐way ANOVA, ns = not significant, ****P < 0.0001. Data are presented as mean, and error bars indicate standard deviation.