Figure EV2. Data related to Fig 2 .

1. Both Fuz transcript and Fuz protein levels were upregulated in the Htttr‐Q92‐expressing HEK293 cells. “Htttr” indicates truncated Huntingtin (Htt), disease protein of Huntington's disease (HD). Lower panel shows the quantification of Fuz transcript and protein expression relative to controls. Error bars represent s.e.m., n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. *P < 0.05, ***P < 0.001.
2. Overexpression of expanded ATXN3tr‐Q78 protein increased the levels of expression of Fuz transcript and Fuz protein in HEK293 cells. “ATXN3tr” indicates truncated ataxin‐3 (ATXN3), disease protein of spinocerebellar ataxia type 3 (SCA3). Lower panel shows the quantification of Fuz transcript and protein expression relative to controls. Error bars represent s.e.m., n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. *P < 0.05, **P < 0.01.
3. The expression levels of Fuz transcript and Fuz protein were induced in SCA3 patient fibroblasts. Lower panel shows the quantification of Fuz transcript and protein expression relative to controls. Error bars represent s.e.m., n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. **P < 0.01.
4. Quantitative RT–PCR was used to determine the expression level of induced neural progenitor cells (iNPCs) specific marker Nestin in fibroblasts and iNPCs. Expression of Nestin was robustly induced in both normal and SCA3 iNPCs. Error bars represent s.e.m., n = 3.
5. Immunostaining of iNPCs using anti‐Nestin (red) antibody, and nuclei were stained with Hoechst 33342 (blue). Both normal and SCA3 iNPCs showed the expression of Nestin protein. Scale bars: 100 μm. n = 3.

Data information: Beta‐actin or beta‐tubulin was used as loading control. n represents the number of biological replicates. Only representative images, gels and blots are shown.Source data are available online for this figure.