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. 2018 Jul 19;19(9):e45409. doi: 10.15252/embr.201745409

Figure 5. Expanded polyQ perturbs the function of YY1 to derepress Fuz expression.

Figure 5

  • A
    Knockdown of YY1 expression upregulated Fuz expression in HEK293 cells. Lower panel shows the quantification of Fuz transcript expression relative to controls. Error bars represent s.e.m., n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. *P < 0.05.
  • B
    Overexpression of YY1 downregulated Fuz expression in HEK293 cells. Lower panel shows the quantification of Fuz transcript expression relative to controls. Error bars represent s.e.m., n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. *P < 0.05.
  • C
    5‐Azacytidine treatment induced the expression of Fuz in HEK293 cells. Lower panel shows the quantification of Fuz transcript expression relative to controls. Error bars represent s.e.m., n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. *P < 0.05.
  • D, E
    Overexpression of YY1 promoted the hypermethylation (D) while knockdown of YY1 expression led to the hypomethylation (E) of Fuz +117/+347CpG. Lower panel shows the quantification of DNA methylation level of Fuz +117/+347CpG relative to controls. Error bars represent s.e.m., n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. *P < 0.05.
  • F
    Overexpression of YY1 promoted the hypermethylation of Fuz +117/+347CpG in the untransfected or ATXN3tr‐Q27‐expressing HEK293 cells. The ATXN3tr‐Q78‐mediated hypomethylation of Fuz +117/+347CpG was rescued by YY1 overexpression. Lower panel shows the quantification of DNA methylation level of Fuz +117/+347CpG relative to controls. Error bars represent s.e.m., n = 3. Statistical analysis was performed using one‐way ANOVA followed by post hoc Tukey's test. *P < 0.05.
  • G
    Overexpression of YY1 suppressed the ATXN3tr‐Q78‐mediated Fuz induction, JNK phosphorylation and caspase‐3 cleavage in HEK293 cells. n = 3.
Data information: beta‐actin or beta‐tubulin was used as loading control. n represents the number of biological replicates. Only representative gels and blots are shown.Source data are available online for this figure.