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. 2018 Aug 20;19(9):e45889. doi: 10.15252/embr.201845889

Figure EV1. Rescue of TMEM41B KO with sgRNA‐resistant TMEM41B.

Figure EV1

  1. H4 Cas9 TMEM41B KO and NT control cells were probed for p62 and NDP52 by immunostaining and imaged with an automated CV7000 confocal microscope. Scale bar: 20 μm. Staining intensity was quantified with ImageJ and depicted as mean ± SD (n = 4 independent experiments) with paired t‐test values.
  2. HeLa Cas9 cells were infected with sgRNAs targeting TMEM41B or NT control alongside lentiviruses expressing sgRNA‐resistant Myc‐tagged TMEM41B, treated with 500 nM AZD8055 or vehicle control for 24 h, and analyzed by immunoblotting 7 days post‐infection.
  3. H4 Cas9 cells were infected with sgRNAs targeting TMEM41B or NT control alongside lentiviruses expressing sgRNA‐resistant untagged or Myc‐tagged TMEM41B, treated with 500 nM AZD8055 or vehicle control for 24 h, and analyzed by immunoblotting 7 days post‐infection.

Source data are available online for this figure.