Fig. 5.

Spatial distribution of TFV and TFV-DP in colorectal tissue sections of two research participants received oral TFV as TFV disoproxil fumarate. (A and D, upper panel) H&E stain exhibiting tissue anatomy and orientation. Representative MALDI MS ion images of (B and E, upper panel) TFV and (C and F, upper panel) TFV-DP revealing the heterogeneity in their distribution. In contrast, lipid species, (A and D, lower panel) PC (34:1), (B and E, lower panel) PC (16:0/OH), and (C and F, lower panel) PC (36:2) showing relative homogeneity in their distribution. Spatial resolution for MALDI MS ion images was 50 μm. Red represents the highest signal intensity (100%), blue represents the lowest signal (0%) of the ion of interest. Scale bar, 1 mm.