Fig. 4.

p66^Shc has a pivotal function in hyperglycaemia-, but not hyperlipidaemia-induced atherosclerosis. a. Experimental design of bone marrow transplantation experiments. b. Transplantation of p66^Shc−/− deficient bone marrow markedly reduces hyperglycaemia-, but only slightly hyperlipidaemia-induced atherosclerosis. Representative images showing Oil Red O staining of aortic roots (left panel) and dot plot summarizing plaques size (right panel). c. Dot plots summarizing results of necrotic core area (left panel), fibrous cap thickness (middle panel), and frequency of ruptured shoulders (right panel). d. Representative images showing immunofluorescence staining of macrophages (MOMA-2, green; DAPI nuclear counterstain, blue) within lesions (left panel) and dot plot summarizing data (right panel). Cont: normoglycaemic ApoE^−/− mice with normal chow diet; DM: hyperglycaemic ApoE^−/− mice; HFD: ApoE^−/− mice with high-fat diet (HFD). Data shown as dot plots represent mean ± SEM of 6–10 mice per group (b–d); size bars: b, d: 20 µm; **P < 0.01; b–d: two-way ANOVA with Bonferroni-adjusted post hoc comparison of ApoE^−/− (Cont, HFD, DM) versus p66^Shc−/− ApoE^−/− (Cont, HFD, DM respectively) recipient mice