Fig. 6.

Effects and defensome response of Oikopleura dioica embryos treated with extracts of different diatom species. a Whole mount in situ hybridizations with Gclm on DMSO-control embryos and embryos treated with extracts of S. marinoi at different concentrations suggest that other PUAs than DD (e.g., heptadienal and octadienal) also activates a quick response of the defensome system. Scale bar 50 µm. b Embryo phenotypes observed after treatments with extracts of S. marinoi (Sm), C. affinis (Ca), C. calcitrans (Cc) corresponds to the same observed after DD-treatment (Fig. 1). Extract of P. minimum (Pm), a dinoflagellate that does not produce aldehydes, was used as additional control. Statistical analyses and individual data plotting are provided in Supplementary Fig. 2