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. 2018 Sep 5;50(9):116. doi: 10.1038/s12276-018-0141-y

Fig. 1. SpAdK expression increases capsular polysaccharide (CPS) levels.

Fig. 1

a Protein levels of SpAdK from lysates of S. pneumoniae D39 WT and TTL01 strains under various concentrations of fucose. b, c Total CPS from whole-cell lysates of S. pneumoniae D39 WT and TTL01 strains under various concentrations of fucose was isolated, and the amount was measured using (b) the phenol–sulfuric method and c ELISA after cross-reacting with the type 2 CPS antiserum. All samples were assayed in triplicate, and each assay was repeated three times. Significant differences were analyzed by one-way ANOVA (*P ≤ 0.05 and ***P ≤ 0.001). d FITC-labeled CPS of S. pneumoniae was visualized by confocal microscopy. The data are representative of three independent experiments