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. 2018 Sep 5;50(9):116. doi: 10.1038/s12276-018-0141-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Pneumococcal lysates were subjected to Phos-tag^TM SDS-PAGE and western blotting with anti-Cps2D to compare phosphorylation levels of Cps2D between S. pneumoniae D39 WT and TTL01 strains. The data are representative of three independent experiments. b ImageJ was used for densitometry analysis and to determine the differences in phosphorylated Cps2D levels between D39 WT and TTL01 strains. One-way ANOVA was used for statistical analysis (*P ≤ 0.05 and **P ≤ 0.01)