Table 1.
Bacterial strains and plasmids used in this study
| Strains/plasmids | Relevant characteristics | Antibiotic resistance | Reference |
|---|---|---|---|
| Strains | |||
| S. pneumoniae | |||
| D39 | Encapsulated, type 2 | 26 | |
| TTL01 | D39 PfcsK::adk carrying an ermAM cassette | ERY | 3 |
| CP1200 | Non-encapsulated derivative of Rx1 malM511-str1 | 25 | |
| TTL04 | CP1200 PfcsK::adk carrying an ermAM cassette | ERY | 3 |
| R6 | Non-encapsulated derivative of D39, no cpsBCD | 26 | |
| E. coli | |||
| BL21(DE3) | gal (cI ts857 ind1 Sam7 nin5 lacUV5-T7 gene 1) | Novagen | |
| Rosetta2 | Expresses seven rare tRNAs | Novagen | |
| Plasmids | |||
| pET32b | 5899 bp; E. coli plasmid | AMP | Novagen |
| pCPS01 | His-tagged cps2B in pET32b | AMP | This study |
| pCPS02 | His-tagged cps2C in pET32b | AMP | This study |
| pGST-parallel 2 | 5038 bp; E. coli plasmid | AMP | 55 |
| pHis-parallel 2 | 5517 bp; E. coli plasmid | AMP | 55 |
| pGST-ADK | GST-tagged SpAdK in pGST-parallel 2 | AMP | This study |
| pCPS03 | His-tagged cps2D in pHis-parallel 2 | AMP | This study |
| pCPS04 | His-tagged cps2D-3Y3E in pHis-parallel 2 | AMP | This study |
| pCPS05 | His-tagged cps2D-GA-KK in pCPS03 | AMP | This study |
| pCPS06 | His-tagged cps2D-GA-KK in pCPS04 | AMP | This study |
ERY erythromycin, AMP ampicillin