Fig. 4.

Scatterplots of normalized Raman peak intensity and normalized gene expression for genes related to three modes of action, cell wall (a), DNA gyrase (b), and protein synthesis (c). Wavelengths that helped identify these modes of actions were selected and associated to genes that may contribute to these antibiotic resistances. On each scatter plot, each point represents a strain for which the gene expression was measured by microarray, and the spectral intensities were averaged from 16 population (laboratory-evolved strains) or 48 population (parental strain). A linear fit was applied to each scatterplot, and the Pearson correlation value R is displayed on each graph. Two-tailed test and FDR assessed that correlations greater than 0.601 in absolute value were significant (|R| > 0.601, FDR p < 0.05)