Fig. 5.

Regulation of Hummr by Ad4BP/SF-1. a The expression of Hummr in Ad4BP/SF-1 knockdown or control Y-1 cells was examined by qRT-PCR. *p < 0.05. n = 3. b Ad4BP/SF-1 ChIP peaks at the Hummr gene in Y-1 and Leydig cells in the presence or absence of cAMP are shown. The ChIP peaks are enclosed by a red square. c Accumulation of Ad4BP/SF-1 to the ChIP peak of Hummr gene was confirmed by ChIP-qPCR. *p < 0.05. n = 3. a, c Average values and SDs are shown. The average value of the control was normalized to 1.0. d A luciferase reporter gene, Hummr-Luc, was constructed with the genome fragments where Ad4BP/SF-1 was accumulated (gray square) and the promoter of Hummr gene (white square). Ovals represent potential Ad4BP/SF-1-binding sites. The reporter was transfected into Y-1 cells with siAd4BP/SF-1 or siControl. Average values and SDs of the luciferase activities are indicated. The average value in the absence of siRNA was normalized to 1.0. *p < 0.05. n = 3. e Effects of Ad4BP/SF-1 overexpression on Hummr-Luc was examined. The reporter was transfected into HeLa cells with increasing amounts of the expression vector for Ad4BP/SF-1. Average values and SDs of luciferase activities are indicated. The average value for pGL3 basic in the absence of the expression vector was normalized to 1.0. n = 3. f Levels of mitochondrial cholesterol in control, Ad4BP/SF-1 knockdown, and Hummr-overexpressing Ad4BP/SF-1 knockdown Y-1 cells. Average values and SDs are shown. *p < 0.05. n = 5