Fig. 5.

RapP turns host cells into cheaters. a Cheating assays in NCIB 3610, except inoculating ∆pBS32 swarms with increasing proportion of wild-type cells instead of ∆srfAA. OD₆₀₀ readings of total swarm yield, normalized to the value of 0% wild type. Relative fitness of the wild-type strain when mixed with ∆pBS32 with or without rapPphrP added in to the chromosome. The only swarm yields significantly different from 0% wild type were from wild type+∆pBS32 (all P < 0.0001). The wild type + ∆pBS32 fitness measurements of 10% and 33% were statistically significant (P < 0.0001 and 0.0008), as was the wild type + addback 10% (gray triangle) from both ∆pBS32-alone (P < 0.0001) and from wild type + ∆pBS32 10% (black circle, P = 0.0042). b Cheating assays with PS-216 or NCIB 3610 ∆pBS32 combined with increasing proportion of cells containing rapPphrP inserted into the chromosome (+rapP strain). All swarm yields were statistically significant except ∆pBS32 33% (P = 0.507), as were fitness values of PS-216 10% (P = 0.0080) and ∆pBS32 10% and 33% (P < 0.0001 and 0.0009). All points in figure are averages of at least two biological replicates, error bars represent SEM.