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. 2018 Jun 29;1:84. doi: 10.1038/s42003-018-0089-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — FISH mapping of centromeric and pericentromeric probes on pachytene chromosomes of O. granulata and phenotypic characterization of its pachytene chromosomes. a Immunodetection of rabbit antibody against rice CENH3 on pachytene chromosomes of O. granulata. b FISH identification of precipitated DNA isolated by ChIP using rice anti-CENH3 antibodies. c Dual color FISH of GCS1 cloned from ChIP DNAs and RIRE2. GCS1 was labeled with digoxigenin-dUTP (magenta), while RIRE2 was labeled with biotin-dUTP (cyan). d Immuno-FISH detection of RIRE2. Rice anti-CENH3 antibodies were used to label centromeres. e Pachytene chromosome morphology of O. granulata. Arrows indicate the 12 centromere constrictions on each pachytene chromosome of O. granulata. f Pachytene chromosome morphology of O. sativa. Chromosomes were counterstained with DAPI. Scale bars = 5 μm